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primer design using 16s rRNA - (Oct/04/2012 )

I got about five to six unknown bacteria and I want to identify the genus and species of that bacteria. So, I want to use the 16s rRNA to design the primer. As far I know we need the universal primer to identify the bacteria. But how to design the primer ? Is there any method or software that can help to design it?

-juanna-

http://openwetware.org/wiki/Bacterial_species_identification

-phage434-