Primer Design HELP! - (Sep/23/2012 )
Hi guys,
Is there a posibility of making primers that are designed for amplification of Beta Actin Gene in both Cauliflower and Chicken liver DNA?
Please read the attachment >.<
-Eriel Lualhati-
It is possible if the two species happen to share a common sequence for beta actin gene.
-pcrman-
AFAIK there is no known sequence for beta actin gene of Brassica oleracea.
Don't see any attachement.
-Trof-
align the two sequences and hope that both the 5' and the 3' are conserved for the first 15-20 bp and last 15-20 bp; this if you want the full sequence start to end
-ascacioc-