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Primer Design HELP! - (Sep/23/2012 )

Hi guys,
Is there a posibility of making primers that are designed for amplification of Beta Actin Gene in both Cauliflower and Chicken liver DNA?

Please read the attachment >.<

-Eriel Lualhati-

It is possible if the two species happen to share a common sequence for beta actin gene.

-pcrman-

AFAIK there is no known sequence for beta actin gene of Brassica oleracea.
Don't see any attachement.

-Trof-

align the two sequences and hope that both the 5' and the 3' are conserved for the first 15-20 bp and last 15-20 bp; this if you want the full sequence start to end

-ascacioc-