Expressing protein in non-E.coli - (Sep/22/2012 )

For expressing protein in non-E.coli cells which plasmid can be used? thanks.

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Well you need to know first what cells you're going to use, then you can find suitable vector.

-Trof-

Trof on Sat Sep 22 10:31:23 2012 said:

Well you need to know first what cells you're going to use, then you can find suitable vector.

Salmonella typhimurium. pcdna3.1 may be compatible but I need BamHI-SalI MCS containing vector. I do not need vector constitutive expression in a variety of mammalian cell lines. I'm looking for vector surviving in salmonella. like PET32 in E.coli.

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