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Synthesis of carrier RNA(poly-A) for DNA Extraction - (Sep/19/2012 )

Dear All,

My lab is planning to synthesize some carrier RNA (poly-A) to increase the yield of (viral) DNA precipitation. Does the length of poly-A RNA matters? What should be the best length to be synthesized? Thanks all!

-GordonLeung-

GordonLeung on Wed Sep 19 13:20:37 2012 said:


Dear All,

My lab is planning to synthesize some carrier RNA (poly-A) to increase the yield of (viral) DNA precipitation. Does the length of poly-A RNA matters? What should be the best length to be synthesized? Thanks all!


yes it matters 35 to 40 datp is most suitable..

-talha-

Thanks Talha. :D

-GordonLeung-