Preparation of phosphate potassium buffer - (Sep/10/2012 )

I want to prepare phosphate potassium buffer 0.1 M (pH=7.4) , consist of 0.5 mM EDTA. Supposed daily consumption is 80mL.
I have 1M K2HPO4 stock, 1M KH2PO4 and 0.5M EDTA stock. My questions are:

<*>Could i be able to prepare 200mL of above mentioned buffer and use for one week via following method?

0.2mL EDTA 0.5 M + 16mL K2HPO4 1M + 4mL KH2PO4 1M

then dilute to 200mL (with adjusted pH).


2. The prepared buffer should be stored in refrigerator?


Your prompt response will be really appreciated!

No - check your daily volumes vs total for 1 week.

mahroo1356 on Mon Sep 10 17:56:27 2012 said:

what i would do is prepare a 1M stock of kphos, pH 7.4, using the 1M mono and dibasic stocks then dilute an aliquot to 0.1M (adding edta) as needed.

can be if you prefer to prepare a larger than immediately necessary volume. the nice thing about kphos, as opposed to naphos, is that it doesn't crystallize at refrigerator temperatures.

thank you for kind attention