Protein precipitation from RIPA buffer to perform ELISA - (Sep/07/2012 )

Hello,

I have extracted protein from tissue in RIPA buffer. I have got good yeild but I understood from the Kit protocol/ company's technical support that RIPA buffer cannot be used for doing ELISA's. Could anyone please tell me a working protocol to precipitate protein out of RIPA buffer so that I could use it for ELISA's. .

Many thanks in advance.
Surya R

you can dialyze away the problematic components of the ripa.

Thank you. Could you please elaborate or send me a protocol to do it. Thanks in advance

check these web pages for a quick description:

fundamentals of dialysis

and

dialysis faq

thank

mdfenko on Mon Sep 10 16:09:21 2012 said: