Southern blotting - (Sep/03/2012 )

Is there any effect from the presence of EtBr in the agarose gel for the subsequent capillary transfer for Southern blotting protocol ? I usually do EtBr staining after electrophoresis to confirm the restriction digestion of DNA samples.

It doesn't affect the transfer usually. I have always stained my Southern blots with EtBr prior to transfer and never had a problem. It may slightly retard the blotting step, but with the times (overnight) usually used for this procedure, it shouldn't be a problem.

Thanks a lot !