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How many generation of HEK293 cell can undergo? - (Aug/07/2012 )

Hello to all.
I am working with a HEK293 which is transfected with NLG1. I would like to know how many time I can passage it to a point that the HEK cell may appear problem in expressing the NLG1 or mutation may occur.

-YC32-

It depends on the generation time, and what you are doing to them as to how likely they are to get mutations. In theory, they won't get a mutation but in practice this isn;t true.

However, mutations aside, it is poor cell culture practice to continuously culture cells for extended periods of time as the cell integrity and behaviour changes with increased passage - meaning that they are no longer the cells you thought they were. As a general rule 10-15 passages and you should get up a fresh vial. If you freeze a couple of vials of cells down at early passages, then you can have an almost limitless supply of cells which will be very very similar to the parent line.

-bob1-