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How to check primer sequences using BLAST and other tools? - (Jul/23/2012 )

Hi,
I need help
how do i check if the primers I have are for mouse with BLAST?
I have no idea how to use the link

-nataly-

there is a drop down menu in the middle of the page you need to change that to mouse. It is on human by default.

-Curtis-

You can use splicenter to check your primers http://projects.insilico.us/SpliceCenter/PrimerCheck

To use NCBI blast, go to http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?LINK_LOC=BlastHome, In the primer parameters section, type your primer sequences

You can also use UCSC in silico PCR tool here http://genome.ucsc.edu/cgi-bin/hgPcr?db=hg18

-pcrman-