is outdated blood suitable for PBMC isolation? - (Jul/13/2012 )

Hi, I want to extract PBMC from blood for IFN-gamma ELISPOT assay.

We have outdated in blood center of our hospital. I wonder if these blood is suitable for PBMC isolation?

Or I must use fresh blood to get PBMC?

Thanks!

I think you can use it. People sometimes get them from blood banks