interest protein and total protein ratio range - (Jun/24/2012 )
I would expresse a foreign protein in 293T by transient transfection. I want to know how may the weight rotio range of the foreign protein/total protein be. I failed to find some answers to this question，I wonder if someone would share some experience no matter about what kind of cells. thank you.
-mawson-
It all depends on how good the transfection is and how strong the promoter is for expressing the protein off the plasmid. I would expect that the ratio would be in the range of 1:1,000,000 or lower - often you can't even see a change in band intensity (of the correct size) on a coomassie or ponceau stained gel/membrane following SDS-PAGE of transfected cells.
-bob1-
bob1 on Mon Jun 25 21:10:36 2012 said:
It all depends on how good the transfection is and how strong the promoter is for expressing the protein off the plasmid. I would expect that the ratio would be in the range of 1:1,000,000 or lower - often you can't even see a change in band intensity (of the correct size) on a coomassie or ponceau stained gel/membrane following SDS-PAGE of transfected cells.
-mawson-