DMSO and MTT assay - (Jun/07/2012 )

Dear all,

Thank you for all reply.

Today, I'd like to ask about DMSO sulution that use for mtt assay in the final step. I'd like to know that which concentration of DMSO use to dissolved formazan crystal product before measuring the absorbance.

How to prepare the MTT reagent? For the best solution. It should be filter-sterlized or not. I should place it overnight for completely dissolve or not.

best regard,

ultra_man

did you got your answer yet?