Problems with PMA treatment - (Jun/04/2012 )

Hi Everyone,

I was hoping I could get some tips on treating cells with PMA. I got a really robust effect in our neuron cell culture with treatment with PMA a couple of times. However, I'm not getting this effect anymore. I tried new PMA, higher and lower concentrations, longer time points and different culture batches. I was wondering if PMA is sensitive to things like temperature and medium composition. Is PMA poorly soluble at low temperatures and does it need to be warmed up and mixed very well before diluted in solution? Any tips on using PMA will be helpful.

Thanks,

are you treating the same cells over a period of time with PMA, or is your non-effect observed for a new batch of cells?