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Fly cross help - (May/07/2012 )

Hello,

I would like to make a fly line that will express my protein of interest (MPI) through theGal4-UAS system. I have a fly line with the UAS-MPI and another fly line with Actin-Gal4 switch. I would like to make one line homozygous for UAS-MPI and Actin-Gal4 switch so I can induce expression of MPI at any point by feeding flies with RU486. I would also like to follow the crosses with a clear phenotype, not by doing PCR to follow them.

These are the genotypes of the 2 fly lines I have:

y<1> w<*>; P{w<+mC>=UAS-MPI.P}2 and

P{ry<+t7.2>=hsFLP}22, y<1> w<*>; P{w<+mC>=UAS-GFP.S65T}Myo31DF; P{w<+mC>=Act5C(-FRT)GAL4.Switch.PR}3/TM6B, Tb<1>

I can get other lines with various balancers to do crosses with if needed.

Thank you for any help you can provide.

-mcb56-

But what is the question?

-hobglobin-

The question is how do I make the UAS-MPI; Actin-Gal4 switch fly?

-mcb56-