OVERDILUTION OF TBE BUFFER - (May/01/2012 )
HI ALL
I wanted to make a 1x TBE solution from a 1 Litre bottle of Lonza Accugene 10x TBE buffer. However, instead of adding 9 Litres of milliq water to the buffer I added 10-11 litres of milliq water.
I wanted to know two things
can i use this TBE as it is to make DNA gels for genotyping
or
could i just get another bottle of 10x TBE add it to my existing stock then bring up to 20 litres with milliq to even out my error
thanks
-helencollins560-
It will probably work as is. You should make your gels with the same buffer.
You can fix this as you suggest by making additional buffer, if you would like.
-phage434-