lymphocytes culture - (Apr/23/2012 )
Hello everyone,
by lack of money in my laboratory we have no incubator CO2.and my question is: can we do in a lymphocytes culture in incubator without CO2.
Thank you.
-sagni2002-
Hello, sagni2002. I used to culture lymphocytes in sealed sterile (30ml) universal tubes (in case you have a problem with culture flasks as well), without CO2.
-cell slave-
cell slave on Wed Apr 25 13:04:24 2012 said:
Hello, sagni2002. I used to culture lymphocytes in sealed sterile (30ml) universal tubes (in case you have a problem with culture flasks as well), without CO2.
Cell slave is correct, you can grow all cells without CO2 in a selaed tissue culture flask. However you must use a media that has 25mM HEPES buffer AND refed constantly to replenish oxygen
Kindest regards
Uncle Rhombus
-rhombus-
hello,
is what it is like if I make my cultures in microplates.
Tlank you.
-sagni2002-