pH rise upon filtration - (Apr/20/2012 )
Example:
pH is adjusted to 7.00. After filter sterilization, the pH becomes 7.10-7.30.
My questions:
Why does the pH rise upon filtration? What's the mechanism behind it?
Does the pH rise only in medium containing sodium bicarbonate? Does it also rise in PBS (Na-Bicarb free)?
Any thoughts are welcome.
-newbie99-
Some of it probably depends on the filter type, but I suspect that the majority will be because of absorption of CO2 from the air during the collection part.
-bob1-
absorbing co2 would lower the pH.
sterile filtration will also somewhat degas the solution. the removal of co2 may, therefore, be the cause of rising pH.
-mdfenko-