Calu-3 cell line do not grow properly - (Mar/01/2012 )
I have a problem... A collaborative lab send me the cells by mail as had send some other cell lines. But this Calu-3 appears to have a slow growth. The flask is in the incubator for more then 2 weeks and there is only less then 50% confluence. The cells divide but most of it remain detached in the medium and are removed during the medium replacement... ![]()
The medium used is the same that in the other lab. DMEM:F12 with 10% FBS, 1%L-glutamin, 1% NEAA and 100 mg:
ml penicillin/streptomycin. How long did normally Calu-3 cell line to get confluent?
Can anybody help me? Please...
-anasus-
Any pretreatment of the flasks? You might want to coat the flasks before culturing your cells. Ar you using non adhesive flasks?
-Salvador-