Has anyone ever had a large insert when subcloning? Mine is 4.3kb? - (Feb/21/2012 )

Have been trying to clone this for 8 weeks.. the insert is 4.3kb and the vector is 5.3kb! No luck...

"This." What could you possibly expect us to know about "this." My sympathy is all I can offer.

oh dear... clone with a large insert (4.3kb).. and a vector which is 5.3kb...