cell plating density - (Feb/10/2012 )

hi everyone,
I am kind of confused with the number of cells needed to plate in a well plate..
data i have:
cell/ mL : 6.175 X 10^5
suspension volume (Trypsin + FBS) : 4 mL
I need to add 100 micro L of 10 ^ 5 cell/mL for the above.. how do i do that?
or
If i need to add 5 X 10^4 cells per well in a well plate..how do i do it?

please respond ASAP

Regards.

you have 6x 10^5cells/ml and you need 1x 10^5cells/ ml cells, so your stock is 6 fold concentrated.

Dilute the cell suspension by 6 fold (1 ml of stock + 5 ml media should do it) and you should have the right density. and add 100ul into each well.

Does this make sense?

yup...i got it.... have done it already thanx for the reply