Wacky BioAnalyzer Results - (Jan/04/2012 )
could be due to genomic DNA contamination that causes rise and fall in baselines ...prefered in line 7-11. These baseline inregularities are due to the presence of longer ssDNA fragments of gDNA.
How did you isolate your RNA? ...is a gDNA contamination possible? Have you run your sample on a agarose gel before?
Regards,
p
If you had problems to get a good ladder profile, than you got a general problem.
Your ladder profile sugest some problems in the gel, or the pressure.
Next idea: The Syringe clip should be the upper position (Nano and Pico Chips)
Yep, the clip is in the upper position. I was concerned that pressure may the issue, but when the clip is released