mir30-based shrna cloning-mismatch - (Dec/23/2011 )

Dear All,

I cloned a mir-30 based shrna on a lentiviral vector recently. I submitted for sequencing and found out that there is some mismatches on sense and antisense sequences but not mir30 context and loop sequence.

Any ideas why it is like that and how to avoid it?

Thanks

-Ambinlab-

mismatches on sense and antisense sequences but not mir30 context and loop sequence.

It is not clear to me what you mean by "not mir30 context and loop sequence". Whether it is intended for a few mismatches between the sense and antisense sequences in the shRNA?

-pcrman-