smear bands in western blot - (Dec/13/2011 )
mdfenko on Wed Dec 14 20:54:38 2011 said:
that "smearing" may be due to aggregation of your protein
To be honest, I don´t think so, since I am expecting a very very low expression level. However, it is worthy considering all possibilities. May you tell me how can I avoid aggregation, please?
-myxobacteria-
depends on the protein. some aggregate readily and require some additives to prevent aggregation.
you may also be boiling the sample in sds buffer to little or too much. you can reduce the temperature of the sample heating to about 65C and incubate for 10-20 minutes.
-mdfenko-
Ok! I will try your piece of advise. Thank you very much
-myxobacteria-