Cell fractionation - (Oct/14/2011 )

Does anyone have a good protocol for cell fractionation so you can get cytoplasmic, nuclear and chromatin fractions?

http://www.uni-kl.de/wcms/fileadmin/tierphys/publikationen/Guillemin_Becker_Proteomcs_2005.pdf


Isabelle Guillemin et al., 2005
(A subcellular prefractionation protocol for minute amounts of mammalian cell cultures and tissue)

Thanks, still need a protocol for chromatin isolation from nuclei