competent cells - (Oct/05/2011 )

Hi All,

for Inoue method of Competent cell preparation how to add MnCl2.4H20 while making the Transformation Buffer? Is it to be added sepaartely or in any other form?

please help.thanks in advance.

MnCl2 * 4 H2O (sterile) need to be added at the end, after the buffer was autoclaved.

In which case, it is no longer MnCl₂ .4H₂O, but Mn⁺, Cl^- - the .4H2O is only for crystalline forms which are hydrated.

You can also just filter sterilize the whole lot.