Staining Cells in Suspension - (Sep/18/2011 )

Hi all

Are there any protocols available for staining cells which can be done entirely in suspension (i.e. never smeared)? I would like prefer something with similar results to H&E staining.

Thank you for your time,

Daniel

trypan blue for cell viability others for flow cytometry

dgprotocol on Sun Sep 18 22:28:30 2011 said:

How would you look at them if they don't make it onto a slide at some point?

I am only familiar with trypan blue. You use a hemocytometer...live cells you will only see outline...dead cells will stain darkly with blue color. You will have to do research to check out reagents used for flow cytometry.

I'll take pictures of them flowing through a microchannel.

To reword my question, would it be possible to adapt an H&E staining protocol to cells which are suspended. Could I simply exchange the solutions about the cells in suspension (spin/resuspend)? Also, which steps are most critical for good Hematoxylin staining? I don't seem to be getting nuclear definition.