Multiplex PCR - (Sep/07/2011 )

Hi,

here's a presentation of the problem with the aspecific product,

greetz

I'm not expert in Real-time PCR, however, I do find that your CT value is quite high, >30
I can see there are some possible primer dimmers in your melting curve in your last slide: is this what you mean by "amplify other products"?

yes, but the strange thing is that if you use the primerpair seperated you dont see this product. Probably primer heterodimer between the 2 primerpair, like you say