what's the function of ROX reference dye? - (Aug/09/2011 )
what's the function of ROX reference dye?
And what's wrong with the melting curve?
pls watch the pics,
I choose a well,the No.5 sample,show u the melting curve.Why do the rox dye have a peak? Is it the primer dimer?
and the 3.jpg is the total reasult.
thx!
ROX is used to normalize the signal of your qPCR reactions across the plate. The idea is that if there is more reaction volume in one well compared to the other, or the amount of light captured in one well is higher than another, assuming the reactions across all wells are the same, the amount of ROX will be used by the instrument to normalize this kind of variation across the plate.
From what I can see in your images it seems like you have contamination in your well.
ivanbio on Wed Aug 10 02:44:05 2011 said:
ROX is used to normalize the signal of your qPCR reactions across the plate. The idea is that if there is more reaction volume in one well compared to the other, or the amount of light captured in one well is higher than another, assuming the reactions across all wells are the same, the amount of ROX will be used by the instrument to normalize this kind of variation across the plate.
From what I can see in your images it seems like you have contamination in your well.
thx