Combination of secondary antibodies - (Aug/05/2011 )

Ciao!

I just read something and I think authors made a mistake, but I'm not sure: can you combine a FITC-labeled Goat anti-Rabbit Ab with a TRITC-labeled Rabbit anti-Mouse Ab? Won't they react with each other, giving false positives as a result? So, one might think he has co-localizes two proteins, but actually it's just the two secondary antibodies reacting? Or is the fluorescent marker preventing binding?

Greetz,
Kim

you're correct. the anti-rabbit should interact with the anti-mouse. maybe the authors stripped between probes?

mdfenko on Fri Aug 5 14:43:52 2011 said:

Ciao!

Thanks! I thought so already, but wanted to check! If they did the stripping, then they certainly didn't mention anything about it? Strange.

Bye!
Kim

P.S.: love your quote!