Keratinocytes-urgent - (Jul/22/2011 )

Hi everyone!

Could you tell me how many passages can I do with keratinocytes without compromissing their response to stimulus.
Can I freeze keratinocytes at passage number 40??

Thank you all for your attention.

passage 40 is getting up there. I would not be surprised to find that they have changed significantly by that time.

Hi!
Thank you for your reply.