effect of DNA isolation and storage on methylation - (Jul/21/2011 )

Hi All,

Im new to methylation studies and hope to learn more about methylation.
i want to know if the isolation procedure of DNA has an impact on methylation of genes?
should i use column purification kits or can i even used standard phenol-chloroform method for DNA isolation?
Also can anyone tell me if storage of the isolated DNA in -20C can alter the actual CpG pattern of the genes?

thanks in advance. :rolleyes:

-genie-

genie on Fri Jul 22 04:14:03 2011 said:

All,

Im new to methylation studies and hope to learn more about methylation.
i want to know if the isolation procedure of DNA has an impact on methylation of genes?
should i use column purification kits or can i even used standard phenol-chloroform method for DNA isolation?
Also can anyone tell me if storage of the isolated DNA in -20C can alter the actual CpG pattern of the genes?

Nothing will change the methylation status of your DNA, neither any isolation procedure nor your storage conditions.

Best,

MoB

-MoB-