Stability of recombinant EGF after reconstitution stored @ -20 - (Jul/19/2011 )
Hi
I have to treat my cells with EGF for my experiment. The EGF that we have in the lab is already reconstituted to a certain concentration. The tubes don't have any info about when they were made so I have no idea how long they've been sitting around at -20. I have used it for few of my experiments....but I get different results with replicate experiments. I am thinking EGF we have might be suspect. I have no idea where it was purchased from originally and few vendors mention that shelf life after reconstitution might be anywhere from few months upto a year. Was wondering if anyone had any idea about this. Any help is much appreciated Thanks
-pm1234-
pm1234 on Tue Jul 19 14:02:38 2011 said:
Hi
I have to treat my cells with EGF for my experiment. The EGF that we have in the lab is already reconstituted to a certain concentration. The tubes don't have any info about when they were made so I have no idea how long they've been sitting around at -20. I have used it for few of my experiments....but I get different results with replicate experiments. I am thinking EGF we have might be suspect. I have no idea where it was purchased from originally and few vendors mention that shelf life after reconstitution might be anywhere from few months upto a year. Was wondering if anyone had any idea about this. Any help is much appreciated Thanks
Hola in the web pg of BDbioscience there are recommendatios to store disolved EGF. I hope it serves to you. Buena suerte
-protolder-