Protein stability at room temperature - (Jul/14/2011 )

Dear Friends

I am a new student in biology field. So i have a stupid question in my mind....can anyone please let me know that if protein samples are kept out of ice while working in the lab by mistake for 2/3 minutes whether there is a chance of protein breakdown or degradation??

How long is it OK if kept out of ice??

G1R2

The answer is - it depends on the protein.

K.B. on Thu Jul 14 17:49:32 2011 said:

Thanks a lot KB....I really appreciate your prompt response...

The sample is nuclear extract sample and I am looking for all the glycosylated proteins and Thrombospondin 1..Waiting eagerly for your response....

I don't know temperature stability of a protein I was working on for last couple of years and you expect me to know yours?
Check the literature data.