mg to mg/ml - (Jul/13/2011 )

I have 25g of dry powder that I need to convert to a 10mg/ml solution.
can anyone help me out in this

harry348 on Wed Jul 13 21:13:46 2011 said:

you need 10mg per ml... so if you take 10mg and dissolve this in 1ml .. you have 10mg per ml..

Now the thing is: you have 25 gram... so how does 25 gram corrolates to the volume?

10mg ======> 1 ml
25 mg =====> 1 ml x 2,5 = 2,5ml (multiply by 2,5 because you went from 10 to 25 mg)
25.000mg (25g) =====> 2,5ml x 1000 = ... (multiply 1000 because you go from 25mg to 25.000mg)


Its basic math...

think about it.. see if you understand it.

Hi Veteran,

please help me as well. I need to make a buffer of this proportions (100 mM Tris, pH 7.4, 0.1 M KCl, 1 mM EDTA, 1 mM PMSF) and dont know exactly how much quantities of each reagent should be mixed..I think its a basic technique as well. Thanks

If I were making this up, I would first make (or better, find in the lab) stock solutions:
1M Tris Cl, pH 7.4
1M KCl
500 mM EDTA pH 8.0
100 mM PMSF in isopropanol (careful, highly toxic!)

I would mix 1 liter of my desired buffer by mixing 100 ml Tris, 100 ml KCl, 2 ml EDTA, and 10 ml PMSF solutions, and make the total up to 900 ml. Check the pH, possibly correct, and top off to 1 liter.

Thank you so much for the help....