Dissolving Calcium Chloride in PBS: How to Prevent Precipitation? - (Jul/07/2011 )

Hi there,

I am trying to make PBS containing 100 mM CaCl₂. However, when I dissolve the correct amount of CaCl₂ in the PBS, there is a formation of precipitate. Does anyone know how to prevent this and make 100 mM CaCl₂ PBS?

You are probably forming CaHPO4 which is very insoluble in water... you will have to make a more dilute solution of Ca2+ as you will never be able to overcome this problem.

in addition to bob1: add stock solution of CaCl2 stepwise to PBS under stiring