Stacking gel for DNA PAGE - (Jul/01/2011 )

I am using 6% native PAGE to resolve 200-400bp DNA. I am getting a lot of smear especially when the volume of the sample is high. Does anybody make stacking gel for running DNA on PAGE?

coldlab on Sat Jul 2 01:59:39 2011 said:

i don't think dna would stack the way that proteins do. stacking is dependent on isoelectric point (pI) and the difference in the pHs of the running and stacking buffers.