Repellent of glasses for PAGE - Need tricks for repel the glasses caster (Jun/28/2011 )
Sigmacoat also has a limited lifetime, so old samples may not work well, or at all. Clean glass is an absolute requirement. Scrubbing with alconox or a similar detergent is a good start, but you need to get the glass clean enough so that water sheets and flows over the surface with no beading up.
-phage434-
Inmost sun on Sat Jul 9 20:08:57 2011 said:
I think Sigmacoat is a silane diluted in organic phase; it works for glass but not alox plates (Hoefer); plates must be cleaned, then coated by rubbing a portion with cloth, shortly rinsed with distilled water and dried;
if Sigmacoat does not work in your case it seems to be too diluted for your purpose; you may repeat coating twice or more;
or buy pure silane (Sigma)and dilute with CCl4
Hi, Inmost sun.
Usually, i was practicing the rub with Kimwipes. is it not good enough for rubbing? and what am i supposed to do? should i purchase the new chemical or substitute the glass?
Pardon me, what kind of CCl4 is? i don't ever hear it?
thank you for the guidance.
-tamarine-
phage434 on Sat Jul 9 20:16:06 2011 said:
Sigmacoat also has a limited lifetime, so old samples may not work well, or at all. Clean glass is an absolute requirement. Scrubbing with alconox or a similar detergent is a good start, but you need to get the glass clean enough so that water sheets and flows over the surface with no beading up.
Hi, phage434.
Thank you for the response. Frankly, your hypothesis is good to think. i am gonna check the expired date of my chemical,soon. Would you share me about alconox? is it Extrant (Merck brand)? actually, my lab posses it. and it is a kind of detergent. but, still i cant find the answer conducting these mess.
Your kindness is highly appreciated.
-tamarine-
tamarine on Sat Jul 16 13:02:05 2011 said:
Pardon me, what kind of CCl4 is? i don't ever hear it?
ccl4 is carbon tetrachloride, a solvent.
we used rain-x for our sequencing gels.
-mdfenko-
Thank you mdfenko!!
I gotta do this sooner! Thank you. Thank you. 
-tamarine-
tamarine on Sat Jul 23 11:39:35 2011 said:
Thank you mdfenko!!
I gotta do this sooner! Thank you. Thank you.
Cleaning glasses first with ethanol then with isopropanol also works well.
-andrea massimo-