Freezing density for NIH 3T3 cells - (Jun/22/2011 )

What freezing density is best to use for NIH 3T3 cells?


Thanks.
Labrat612

I don't know about what is best, but we typically freeze a few million cells (btw 5 and 10) in a single aliquot of freezing medium (1ml). Seems to work just fine.

labrat612 on Wed Jun 22 18:46:51 2011 said:

I freeze down 1-3 million cells in 1.5 ml of freezing media. This allows me to resusitate cells into either a T25/T75 TC flask. NIH 3T3 cells are mouse fibroblasts which grow very well....so you might even get away with 500,000 cells/vial.

I rarely count my cells when freezing them down, estimating the cell number depending upon the surface area of the flask.

Hope this is useful.

Kindest regards.

Uncle Rhombus

Thank you all for these suggestions! It is definitely much appreciated!!!