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is it a contamination? help - (Jun/03/2011 )

hello
i'm working with RKO and i obtain this pictures today
i think it could be a contamination but i'm not sure, some people of may lab say it coul also be detrits from the cells
i used a DMEM medium but it wasen't suplemented with l-glutamine aldo this cells require it. could it be the reason of the small dots or is it bacteria?
help me please
Attached File

-martha2-

martha2 on Fri Jun 3 18:03:26 2011 said:


hello
i'm working with RKO and i obtain this pictures today
i think it could be a contamination but i'm not sure, some people of may lab say it coul also be detrits from the cells
i used a DMEM medium but it wasen't suplemented with l-glutamine aldo this cells require it. could it be the reason of the small dots or is it bacteria?
help me please



Seems big for bacteria - do you have any higher magnification pictures? Was your medium cloudy?

-avalon-

hello
i have this picture from 400x but is of bad quality
the medium didn't seem cloudy but i saw this in a 96 well with 100ul of medium.


avalon on Sat Jun 4 01:49:01 2011 said:


martha2 on Fri Jun 3 18:03:26 2011 said:


hello
i'm working with RKO and i obtain this pictures today
i think it could be a contamination but i'm not sure, some people of may lab say it coul also be detrits from the cells
i used a DMEM medium but it wasen't suplemented with l-glutamine aldo this cells require it. could it be the reason of the small dots or is it bacteria?
help me please



Seems big for bacteria - do you have any higher magnification pictures? Was your medium cloudy?

Attached File

-martha2-