What is the difference between Km and Kd - (May/31/2011 )

Hi there,

Just trying to find out what exactly is the difference between the Michealis constant Km and the dissociation constant Kd?

Kind Regards,
LB

In kinase assay, the Michaelis constant Km is the substrate concentration at which the reaction rate is half of Vmax.
dissociation constant is a specific type of equilibrium constant that measures the propensity of a larger object to separate (dissociate) reversibly into smaller components, as when a complex falls apart into its component molecules, or when a salt splits up into its component ions.