Primer,probes dilution - (May/25/2011 )

I have few primers and probes at 100uM/ul concentration with these specific volumes(ul) 746.22, 530.79, 801.26, and probes with 499.76, 337.97,426.92.
I want to dilute them to 50uM (50pmol/ul).

So how can I do them?
Can anyone here help me out

Thanks in advance

harry348 on Wed May 25 12:02:48 2011 said:

use the equation :
C1V1 = C2V2
the stock conc. x the volume you will take from the stock = the working solution conc x total volume you need to reach.

for example you want to prepare 100 ul working solution so :

C1V1 = C2V2
100uM x V1 = 50 uM x 100 ul ------ this will give u V1 = 50 ul
so take 50ul from the stock and add 50 ul d.H2O.

hope this will help !

-nightingale-

Thank you very much.
So you mean this applies for every primer and probe dilution???

-harry348-

harry348 on Wed May 25 20:55:45 2011 said:

Thank you very much.
So you mean this applies for every primer and probe dilution???

Its a general rule yes.


But try to understand it rather then memorise it by heart.

Simple example:


100µM/µl concentration with specific volume 746.22µl.

And you want 50µM/µl.

How to solve this?

Its nothing more then a dilution, have you solved dilutions before?

You need to go from 100µM/µl to 50µM/µl .... what is this? its a 1/2 dilution...
It means you need to have 1 part of the stock solutions for 2 parts in total... So you simple need to add 1 part water or dilutant.

And you can pick any volume of the stock you want, as long as you add the same amount of water to dilute it.

You can use that formula, but try to understand what it means, what you are doing.
Because if you forget the formula or make a mistake in it.. you end up with the wrong asnwer, however, if you understand the concept of diluting, you will never make mistakes.


PS.: check your units too:

100uM/ul concentration with these specific volumes(ul) 746.22, 530.79, 801.26, and probes with 499.76, 337.97,426.92.
I want to dilute them to 50uM (50pmol/ul).

-pito-