Gel Extraction using buffer QG then phenol/chl - Can I? (May/06/2011 )
I have this 500bp PCR product that I need to purify from gel, but I don't want to use column.
I cut the gel which is 2% Normal Agarose Gel, melt the Gel with buffer QG (from Qiagen Gel Extraction Kit) which contains Guanidium Thiochloride
Phenol Chlroform the rest.
Is this possible?
-MyProteinBulliedMe-
MyProteinBulliedMe on Sat May 7 02:47:12 2011 said:
I have this 500bp PCR product that I need to purify from gel, but I don't want to use column.
I cut the gel which is 2% Normal Agarose Gel, melt the Gel with buffer QG (from Qiagen Gel Extraction Kit) which contains Guanidium Thiochloride
Phenol Chlroform the rest.
Is this possible?
why do not you want to use the column?
-chromatin-