stripping of Western blot? - how does it work (Apr/26/2011 )

hi

could anyone let me know stripping of western blot work??

thank a lot

regards

Hola, it releases all the bounded proteins to the membrane, blocking agent as antibodies. Moreover some of the transferred protein too, for that after stripping coulored markers loose intensity. But after stripping you could reprobing the membrane against other primary antibody .Buena suerte