amount of glycerol - (Apr/20/2011 )

I have an enzyme and I want to keep it with glycerol, There is one formula to calculate to amount of glycerol needs to be in the enzyme with require buffer. Does some one know about the formula????

regards

If I remember right lots of enzyme buffers (taq, restriction enzymes) have 50% glycerol. If you meant this...formula I've no idea.

Yes I am putting the 50 % glycerol. But there is some other ways as well.

hobglobin on Wed Apr 20 15:07:38 2011 said:

Hola Adrian Kofst makes a similar question today, look for answers, 50% glycerol in buffer at -80ºC helps to store your protein active for long periods.Buena suerte