help with cell dilution - (Apr/05/2011 )

Hi. This is very simple but I was never good with mathematics o.O

I need to dilute cells to get 200 000 cells in 200µl of medium. Which is the way to calculate that?

Tnx

Use V1C1=V2C2 (where V= volume and C= concentration and 1 and 2 are initial and final respectively) for one suspension to another.

or
V=N/C (where V and C are as above, and N= number of objects you have (in your case cells)) for pelleted cells.

Thanks mate. I´m just getting into lab work so I´m probably asking some questions with very simple answers. I´m a vet on a doctorate but I have just too much lab work before strating animal trials and I fell like Alice in Wonderland But im slowly getting into this, love it more by each day ) Thanks once more, cheers!