Why shouldn't you clone using expression bacteria - (Apr/01/2011 )

...?

-Ahrenhase-

Transformation efficiency is 1-2 orders of magnitude worse. Any preparing the DNA from these strains is difficult and yields poor quality plasmid DNA.

-phage434-

Searching the forum before asking helps: "BL21 strains are recA+ and recombination is common. Second, they are endA+, which means that DNA you purify during a miniprep is often damaged by endonucleases. " http://www.protocol-online.org/biology-forums/posts/29238.html

-Rsm-

I just realized that above answer was given by a user called "phage434". Weird. :ph34r:

-Rsm-

Rsm on Sat Apr 2 19:29:41 2011 said:

I just realized that above answer was given by a user called "phage434". Weird. :ph34r:

And how is that?
-Perneseblue

-perneseblue-

Who'd trust that fellow, anyway.

-phage434-

phage434 on Sun Apr 3 19:59:10 2011 said:

Who'd trust that fellow, anyway.

haha

-adrian kohsf-