western blot - substarate buffer recepie (Mar/26/2011 )
hii every one
pls tell me the recepie of substrate buffer for western blot. I m using HRP conjugate secondary antibody. currently I m using citrate buffer, H2O2 and DAB but it s not giving good result.
Thanking you,
kalndi
-kalindi-
you can add a little nickel to the substrate solution to make it a little more sensitive
-mdfenko-
mdfenko on Mon Mar 28 20:31:07 2011 said:
you can add a little nickel to the substrate solution to make it a little more sensitive
thank u so mauch....
-kalindi-