power supply electrophoresis - (Mar/21/2011 )

if you haven't done it yet, don't. if you run dissimilar gels at the same time then the current will split unevenly and they will run and heat differently.

mdfenko on Fri Mar 25 15:07:47 2011 said:

Hi,
I already run these 2gels, and definitely, the running was different! 10% gel run much better and a faster than that of 12%. I stopped th running of 10% and removed from the chamber. I kept running 10% gel. I transfer both in the same chamber, wet transfert for 1h at 90V. I saw that the transfert was better in 10% gel. also, the marker lanes were not transferred well. I blocked both in blocking solution, wash with PBS tween. I will perform western blot in both and see!!
thanks