Expressing human protein in E.Coli - (Mar/15/2011 )

Thanks chimaera. Just wondering the recombinant plasmid should be isolated from the cloning host and directly transfect into the expression host for the Novagen pTriEx system, am I right?

alantkl on Fri Apr 1 01:59:29 2011 said:

Hola,Yes with the plasmid from cloning strain you can tranfect mammalian cells or insect cells , in this last case for the homologous recombination method with Bsu36I digested baculoviral DNA from Clontech, Orbigen, Pharmingen or bacvector Novagen etc.This plasmid isn´t compatible with Invitrogen systems Bac-to-Bac, Baculo direct, and blue bac buena suerte con la expresión

Gracias amigo