urgent!!! weird Growth in cell line - (Mar/15/2011 )

hi guys we have recently had fungal contamination in our lab however it was thoroughly cleaned and all flasks changed etc etc.....today wehn i was doing regular viewing of my cells i saw something blurry. when i fine focused into the blur i observerd this.

http://img233.imageshack.us/i/dsc00545cn.jpg/

http://img826.imageshack.us/i/dsc00538e.jpg/

http://img135.imageshack.us/i/dsc00541c.jpg/

these pictures are at 100x and 40 x.....

can someone please help me and tell me what it is......
thanks alot.

Sathyen on Tue Mar 15 15:20:28 2011 said:

It looks like the bottom of the plastic flask/dish to me.....i.e. you have not visulaised the cells.

Kindest regards.

Uncle Rhombus.

those are the leftover of liquid on the bottom of your culture dish, not comtamination.

rhombus on Tue Mar 15 16:48:03 2011 said:

i did visualize the cells...but i saw this in the background. i thought it was the flask too.....so i tried to look at a new flask but the new flask wasnt like that. rather it was clear.... the reason i thought it might have been a contamination was because in our microbiology lab where we were conducting sterility check of some media which was clear at first but after about a week (yesterday) i found plaquess that resembled this pattern. thus i got skeptical...

i did visualize the cells...but i saw this in the background. i thought it was the flask too.....so i tried to look at a new flask but the new flask wasnt like that. rather it was clear.... the reason i thought it might have been a contamination was because in our microbiology lab where we were conducting sterility check of some media which was clear at first but after about a week (yesterday) i found plaquess that resembled this pattern. thus i got skeptical...

Sathyen

You have scraped the bottom of flask with your pipette tip and so this beautiful design. Ha ha. I suppose there is nothing to worry if your cell are ok.

Sathyen on Wed Mar 16 03:59:14 2011 said:

looks so much like snow flakes!
could it be crystal of some salt?

Yep, salt crystals from something left to dry on the bottom of the flask. Nothing to worry about, other than it being an indicator that you probably spilled something and didn't clean it up.

How festive!